The diagnosis of Echinococcus granulosus in dogs.
نویسندگان
چکیده
The problem of diagnosing Echinococcus granulosus in dogs has still only been partially resolved, even after the advent of biotechnology. The eggs of taeniid Cestoda are extremely similar, and thus identification by microscopic examination of the faeces is risky and non-specific. For this reason, Echinococcus granulosus was traditionally diagnosed in dogs ante mortem after an arecoline hydrobromate purge. The faeces were examined macro and microscopically to establish if the adult tapeworm or its proglottids were present. Although this method is 100% specific, it is bio-hazardous and time-consuming, requires trained personnel, and its sensitivity varies. In the 1990s copro-antigens were discovered and characterised. These are released by the adult worm in the faeces. This made it possible to use enzyme-linked immune-adsorbent assay (ELISA) for in vitam diagnosis of Echinococcus granulosus. In recent years several PCR protocols have been published on the identification of Echinococcus granulosus DNA from eggs or from adult parasites and new ways of diagnosing this cestode have been developed.
منابع مشابه
Detection of Echinococcus granulosus infection in dogs
Cystic Echinococcosis is an important zoonosis in the sheep rising areas of Iran. To develop a simple and reliable diagnostic method for Echinococcus infection in definitive hosts, E. granulosus polyoclonal antibodies (PolyAbs) were prepared from adult worm in rabbit. A selected PoAb was used for coproantigen detection in faecal samples obtained from animals naturally infected with Echinococcus...
متن کاملEvaluation of Echinococcus granulosus coproantigens by Dot-blotting in dogs
Definitive hosts of the Echinococcus granulosus (E. granulosus ) parasite are carnivores such as dogs,wolves and foxes. Detection of this parasite through faecal examination is not possible. In this study, dotblotting test for E. granulosus -specific coproantigens has been evaluated in dog. Three 2–3-month-old puppies were treated with piperazine and then faecal samples were collected as pre-in...
متن کاملPrevalence of Echinococcus granulosus and other intestinal helminths of stray dogs in Mashhad area, Iran
In order to determine prevalence of Echinoccocus and other intestinal helminths infection one hundred stray dogs were examined in Mashhad area, Khorasan province of Iran. Helminths were collected from intestinal contents obtained at the time necropsy. Ninety one percent of the dogs were infected by different helminths. The prevalence of Echinococcus granulosus was (22%). Eighteen (81%) of inf...
متن کاملPrevalence of Echinococcus Granulosus in Carnivores of Iran: Systematic Review and Meta-Analysis Study
Abstract In this study, the prevalence of Echinococcus granulosus was surveyed by meta-analysis and systematic review. Using main keywords of Echinococcus granulosus, dog and carnivores, we searched standard sites such as Google scholar, SID, Pubmed, Iranmedex, Irandoc, Science direct and magiran. Fifteen reliable articles were reviewed and analyzed by STATA software, Ver 11. From obtained arti...
متن کاملImmunization of dog with proteins under 30 kDa molecular weight of hydatid cyst fluid and protoscoleces of Echinococcus granulosus
The aim of the present study was to assess the immunogenicity of under 30kDa molecular weight proteins of hydatid cyst fluid and protoscoleces in dogs experimentaly Infected with Echinococcus granulosus. Isolation of under 30 kDa proteins performed using Millipore filter. Six dogs were used in three groups: 2 dogs with under 30 kDa proteins of hydatid cyst fluid (Group I); 2 dogs with proteins ...
متن کاملCopro-diagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated DNA sequence.
Diagnosis of Echinococcus granulosus infection in dogs by detecting adult worms recovered post mortem or purged from the intestines after treatment with arecoline is not suitable for mass screening. Large-scale diagnosis by detection of copro-antigens is useful but only with relatively high intensity infections, and only by genus. To provide a more sensitive and specific diagnosis, a polymerase...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Parassitologia
دوره 46 4 شماره
صفحات -
تاریخ انتشار 2004